In vitro interference of Hyptis martiusii Benth. & chlorpromazine against an aminoglycoside - resistant Escherichia coli

Henrique D.M. Coutinho; José G.M. Costa; Edeltrudes O. Lima; Vivyanne S. Falcão-Silva; José P. Siqueira-Júnior

doi:10.25259/IJMR_20091295_566

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Original Article

129 (

); 566-568

doi:

10.25259/IJMR_20091295_566

In vitro interference of Hyptis martiusii Benth. & chlorpromazine against an aminoglycoside - resistant Escherichia coli

Henrique D.M. Coutinho^,, José G.M. Costa^*, Edeltrudes O. Lima^**, Vivyanne S. Falcão-Silva⁺, José P. Siqueira-Júnior⁺

Laboratório de Pesquisa em Produtos Naturais, Departamento de Ciências Biológicas, Centro de Ciências Biológicas e da Saúde, Universidade Regional do Cariri Crato (CE), João Pessoa (PB), Brazil

*Faculdade Leão Sampaio, Juazeiro do Norte (CE), João Pessoa (PB), Brazil

**Laboratório de Micologia Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde Universidade Federal da Paraíba João Pessoa (PB), João Pessoa (PB), Brazil

+Laboratorio de Genética de Microrganismos Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa (PB), Brazil

Reprint requests: Dr Henrique D.M. Coutinho, Universidade Regional do Cariri - URCA; Centro de Ciências Bioló gicas e da Saú de - CCBS Departamento de Ciê ncias Bioló gicas - DCB; Laborató rio de Pesquisa em Productos Naturaia - LPPN. CEP: 63105-000. Crato (CE) Brasil e-mail: hdmcoutinho@gmail.com

Received: 2008-01-31,

Licence

This open access article is licensed under Creative Commons Attribution 4.0 International (CC BY 4.0). http://creativecommons.org/licenses/by/4.0

Abstract

The antibacterial and synergistic activity of the ethanol extract from Hyptis martiusii Benth. was assayed by microdillution. The growth of two isolates of Escherichia coli tested was inhibited by the extract. The minimum inhibitory and minimum bactericidal concentrations (MIC and MBC) values ranged from 512 and >1024 μg/ml for the E. coli 27 and 1024 and > 1024 μg/ml for the E. coli ATCC8539, respectively. A synergism between this extract and all aminoglycosides assayed was demonstrated. In the same form synergism between chlorpromazine and kanamycin, amikacin and tobramycin was observed, indicating the involvement of an efflux system. Extracts from H. martiusii could be used as a source of plant derived natural products with modifying antibiotic activity and these products may interact and affect multidrug resistance systems (MDR) as efflux pumps.