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Effect of Scutellaria falvonoids on KCN-induced damages in rat pheochromocytoma PC12 cells
Reprint requests: Prof. Ya Zhen Shang, Institute of Traditional Chinese Medicine, Chengde Medical College Chengde 067000, P. R. China e-mail: shangyz1018@yahoo.com.cn
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Received: ,
Abstract
Background & objectives:
Cerebral hypoxia is known to be involved in many neurodegenerative diseases such as Alzheimer's and cerebrovascular dementia. The present study was designed to investigate the effects of flavonoids from aerial part of Scutellaria baicalensis Georgi (SSF) on potassium cyanide (KCN) -induced hypoxic cytotoxicity in rat pheochromocytoma cell line PC12, and to understand the probable mechanism.
Methods:
The rat pheochromocytoma cell line PC12 was subjected to hypoxia by 200 μM KCN for 30 min. The cytotoxicity of KCN was assessed by cell viability assay, morphological observation, lactate dehydrogenase (LDH) release, malondialdehyde (MDA) production, and the activities of superoxide dismutase (SOD) and NaM+-K+-ATPase measurements. The effects of SSF on the changes induced by KCN in PC12 cells were detected.
Results:
Treatment of PC12 cells with 200 μM KCN for 30 min increased cell death when compared with control, as assayed by MTT reduction, morphological observation and lactate dehydrogenase release measurement. These cell lesions were accompanied by disorders in SOD and NaM+-K+-ATPase activities as well as MDA production. In contrast, the PC12 cells pre-treated with SSF for 24 h prior to 200 mM KCN exposure have shown protection against hypoxic toxicity. The KCN – induced decreased cell viability and activities of SOD and NaM+-K+-ATPase, as well as increased MDA production were reversed by SSF pre-treatment.
Interpretation & conclusions:
SSF exerted neuroprotections against KCN –induced hypoxic cytotoxicity in PC12 cells and the probable mechanisms involved free radicals and energy metabolism. Our findings may have implications in future in the treatment of neurodegenerative diseases.
