Generic selectors
Exact matches only
Search in title
Search in content
Post Type Selectors
Search in posts
Search in pages
Filter by Categories
Author’ response
Author’s reply
Authors' response
Authors#x2019; response
Book Received
Book Review
Book Reviews
Centenary Review Article
Clinical Image
Clinical Images
Commentary
Communicable Diseases - Original Articles
Correspondence
Correspondence, Letter to Editor
Correspondences
Correspondences & Authors’ Responses
Corrigendum
Critique
Current Issue
Editorial
Errata
Erratum
Health Technology Innovation
IAA CONSENSUS DOCUMENT
Innovations
Letter to Editor
Malnutrition & Other Health Issues - Original Articles
Media & News
Notice of Retraction
Obituary
Original Article
Original Articles
Perspective
Perspectives
Policy
Policy Document
Policy Guidelines
Policy, Review Article
Policy: Correspondence
Policy: Editorial
Policy: Mapping Review
Policy: Original Article
Policy: Perspective
Policy: Process Paper
Policy: Scoping Review
Policy: Special Report
Policy: Systematic Review
Policy: Viewpoint
Practice
Practice: Authors’ response
Practice: Book Review
Practice: Clinical Image
Practice: Commentary
Practice: Correspondence
Practice: Letter to Editor
Practice: Obituary
Practice: Original Article
Practice: Pages From History of Medicine
Practice: Perspective
Practice: Review Article
Practice: Short Note
Practice: Short Paper
Practice: Special Report
Practice: Student IJMR
Practice: Systematic Review
Pratice, Original Article
Pratice, Review Article
Pratice, Short Paper
Programme
Programme, Correspondence, Letter to Editor
Programme: Commentary
Programme: Correspondence
Programme: Editorial
Programme: Original Article
Programme: Originial Article
Programme: Perspective
Programme: Rapid Review
Programme: Review Article
Programme: Short Paper
Programme: Special Report
Programme: Status Paper
Programme: Systematic Review
Programme: Viewpoint
Protocol
Research Correspondence
Retraction
Review Article
Short Paper
Special Opinion Paper
Special Report
Special Section Nutrition & Food Security
Status Paper
Status Report
Strategy
Student IJMR
Systematic Article
Systematic Review
Systematic Review & Meta-Analysis
Viewpoint
White Paper
Generic selectors
Exact matches only
Search in title
Search in content
Post Type Selectors
Search in posts
Search in pages
Filter by Categories
Author’ response
Author’s reply
Authors' response
Authors#x2019; response
Book Received
Book Review
Book Reviews
Centenary Review Article
Clinical Image
Clinical Images
Commentary
Communicable Diseases - Original Articles
Correspondence
Correspondence, Letter to Editor
Correspondences
Correspondences & Authors’ Responses
Corrigendum
Critique
Current Issue
Editorial
Errata
Erratum
Health Technology Innovation
IAA CONSENSUS DOCUMENT
Innovations
Letter to Editor
Malnutrition & Other Health Issues - Original Articles
Media & News
Notice of Retraction
Obituary
Original Article
Original Articles
Perspective
Perspectives
Policy
Policy Document
Policy Guidelines
Policy, Review Article
Policy: Correspondence
Policy: Editorial
Policy: Mapping Review
Policy: Original Article
Policy: Perspective
Policy: Process Paper
Policy: Scoping Review
Policy: Special Report
Policy: Systematic Review
Policy: Viewpoint
Practice
Practice: Authors’ response
Practice: Book Review
Practice: Clinical Image
Practice: Commentary
Practice: Correspondence
Practice: Letter to Editor
Practice: Obituary
Practice: Original Article
Practice: Pages From History of Medicine
Practice: Perspective
Practice: Review Article
Practice: Short Note
Practice: Short Paper
Practice: Special Report
Practice: Student IJMR
Practice: Systematic Review
Pratice, Original Article
Pratice, Review Article
Pratice, Short Paper
Programme
Programme, Correspondence, Letter to Editor
Programme: Commentary
Programme: Correspondence
Programme: Editorial
Programme: Original Article
Programme: Originial Article
Programme: Perspective
Programme: Rapid Review
Programme: Review Article
Programme: Short Paper
Programme: Special Report
Programme: Status Paper
Programme: Systematic Review
Programme: Viewpoint
Protocol
Research Correspondence
Retraction
Review Article
Short Paper
Special Opinion Paper
Special Report
Special Section Nutrition & Food Security
Status Paper
Status Report
Strategy
Student IJMR
Systematic Article
Systematic Review
Systematic Review & Meta-Analysis
Viewpoint
White Paper
View/Download PDF

Translate this page into:

Original Article
145 (
6
); 746-752
doi:
10.4103/ijmr.IJMR_1225_15

Combination of interleukin-10 gene promoter polymorphisms with HLA-DRB1*15 allele is associated with multiple sclerosis

Medical Cellular and Molecular Research Center, Gorgan, Iran
Department of Neurology, 5th Azar Hospital, Golestan University of Medical Sciences, Gorgan, Iran
Department of Neurology, Mazandaran University of Medical Sciences, Sari, Iran
Department of Neurogenetics, Iranian Centre of Neurological Research, Tehran, Iran

Reprint requests: Dr. Majid Shahbazi, Medical Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran e-mail: shahbazimajid@yahoo.co.uk

Licence

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

Disclaimer:
This article was originally published by Medknow Publications & Media Pvt Ltd and was migrated to Scientific Scholar after the change of Publisher.

Abstract

Background & objectives:

Multiple sclerosis (MS) is common in some ethnic groups. Interleukin-10 (IL-10) is a potent anti-inflammatory and immunosuppressive cytokine that may be an important regulator in MS disease pathogenesis. IL-10 promoter includes several single nucleotide polymorphisms and the level of IL-10 expression is related to these polymorphisms. Furthermore, loci within the histocompatibility regions are responsible for susceptibility to MS. The aim of this study was to investigate the association of IL-10 gene promoter polymorphisms and HLA-DRB1*15 allele frequencies with MS susceptibility in an Iranian population.

Methods:

In this study 336 MS patients and 454 healthy controls were included. Genomic DNA was purified from peripheral blood samples by a standard protocol. Genotyping was performed by the sequence-specific primer polymerase chain reaction method.

Results:

IL-10 −1082 G/G and IL-10 −819 C/C genotypes were more frequent in MS patients than healthy individuals. DRB1* 15 allele showed a higher frequency among MS patients compared to controls.

Interpretation & conclusions:

The IL-10 and HLA-DRB1*15 polymorphisms were associated with the susceptibility to MS in Iranian patients. Our results suggest that gene-gene interaction of IL-10 polymorphisms and HLA-DRB1*15 alleles may be important factors in the development of MS.

Keywords

Cytokine
HLA-DRB1*15 allele
interleukin-10
multiple sclerosis
single nucleotide polymorphism

Multiple sclerosis (MS) is the most common demyelinating disorder, mostly affecting young women. According to the previous studies concerning the incidence rate of MS12, Iran was categorized as a low-risk region3. However, a later study showed that the incidence of MS was more than 35/100,000 in Isfahan, Central Province of Iran4. The autoimmune process results in the destruction of myelin sheaths surrounding axons of the central nervous system leading to neurodegeneration and permanent neurologic deficits4. Though the initiating mechanism is not clear, it seems that the activation of pro-inflammatory cytokine cascade, besides dysregulation of anti-inflammatory processes, has an important role in the pathogenesis of the disease5.

Since the incidence of MS is found to be significantly higher in monozygotic twins in comparison to dizygotic twins6, a genetic basis is suggested and the role of human leukocyte antigens (HLAs) is now widely accepted, especially in the case of HLA-DRB1*157. Further, it has been shown that polymorphisms in the genes encoding cytokines may play a role in the susceptibility to MS8 or disease course9. Ramagopalan et al10, in a large cohort study, have shown that HLA-DRB1*15 allele is overtransferred to MS patients, especially from their mothers. In a collaborative genome-wide association study involving 9772 cases of European descent, the identity of the HLA-DRB1 risk alleles was refined in MS patients. It has been confirmed that variation in the HLA-A gene underlies the independent protective effect attributable to the Class I region11. Tuwir et al12 found that Irish individuals who were positive for the HLA-DRB1*15 allele were more susceptible to develop optic neuritis. They also found that people with optic neuritis and HLA-DRB1*15 allele were at higher risk for developing MS.

Interleukin 10 (IL-10) is an anti-inflammatory cytokine mainly produced by macrophages and T-lymphocytes during autoimmune and infectious diseases1314. The progression or recovery of MS seems to have a direct association with the amount of IL-10 production15. The production of IL-10 is regulated by its gene located on chromosome 1q31-3216. The three single nucleotide polymorphisms (SNPs) in the promoter region of IL-10 gene, 1082 G/A, 819 T/C and 592 A/C, have been shown to be in association with high or low production of this cytokine16. Myhr et al17 have reported that −819 and −592 alleles have complete linkage where TA/CC, TT/CC and AA/CC haplotype combinations are formed. It is proposed that these haplotypes determine IL-10 mRNA levels and regulate its production. Mihailova et al18 found significant differences in the frequency of genotypes between MS patients and controls in Bulgarian population. Luomala et al19 demonstrated that these polymorphisms were in correlation with disease severity through the regulation of production of IL-10 but not with susceptibility to disease.

For detecting the genetic role in susceptibility to MS disease, previously we showed the correlation between polymorphisms in the CCR5 (CC chemokine receptor 5), IL-6 (Interleukin 6), IL-2 (Interleukin 2), TNF-α (Tumor necrosis factor alpha) and CD-24 genes with MS20212223. In this study, the frequency of HLA-DRB1*15 allele and SNPs in the IL-10 promoter region were studied among Iranian MS patients and healthy controls to evaluate the possible role of IL-10 gene polymorphism in MS.

Material & Methods

A total of 336 patients and 454 healthy controls were screened for genetic variations in the promoter region of the IL-10 gene and HLA-DRB1*1501 allele in the Medical Cellular and Molecular Research Center, Gorgan, Iran. Sampling was carried out from volunteer patients during 2009-2011. MS patients were diagnosed by expert neurologists based on clinical and paraclinical findings (magnetic resonance imaging, oligoclonal bands in the cerebrospinal fluid and evoked potentials) according to the McDonald's criteria24. The sample size was calculated to have the minimum effect size with at least 80 per cent power and significance of 95 per cent under the dominance model by the Quanto software V-1.2 (University of Southern California, USA) based on frequency of disease. Moreover, the minor allele frequency was chosen to be 10 per cent and a type 1 error level of 0.05.

Control individuals were people referred to Gorgan blood donation centres in Golestan province who were matched by sex, ethnicity and age, and there were no autoimmune or inflammatory disorders in their history. A pretested demographic questionnaire was used and which included sex and age for both groups, age at onset and expanded disability status scale for MS patients. Demographic information of MS patients is shown in Table I. The mean age for MS patients was 36.8±8.6 yr (ranging from 18-62 yr) and for controls was 35.45±6.9 yr (ranging from 21-62 yr).

Table I Demographic data of multiple sclerosis (MS) patients (n=336)

The study was approved by the Ethics Committee of Golestan University of Medical Sciences (No: 100590041313), and written informed consent was obtained from all patients and healthy controls.

DNA extraction and genotyping: Genomic DNA was purified from 10 ml peripheral blood samples by a standard protocol with some modifications25. Genotyping was done by sequence-specific primer polymerase chain reaction (SSP-PCR) method using a Thermal Cycler (Techne, UK) as described previously21. An internal positive control primer pair, which amplifies a conserved region of the human growth hormone gene was sourced from “TIB MOLBIOL” company (Berlin, Germany) and was included in every PCR reaction mix. The sequence of the primers is shown in Table II.

Table II Primer sequences used for the sequence-specific primer genotyping method

After PCR, the electrophoresis was performed in 2 per cent agarose gel (Merck, Germany) and PCR product bands were visualized by a gel documentation system (UVITEC, UK). The HLA-DRB1*1501 genotyping was carried out as previously described26. The genotyping results were evaluated by the presence or absence of an allele-specific PCR product.

Statistical analysis: Statistical analysis was performed using SPSS 16.0 software (SPSS Inc., Chicago, IL, USA). The possible differences in genotype and allele frequencies were assessed by the Pearson's Chi-square test, and the risk associated with genotypes/alleles was calculated as the odds ratio (OR) with 95 per cent confidence intervals (CIs). A multiple logistic and linear regression analysis was performed to evaluate possible associations between the variables. The estimation of haplotype frequency and the analysis of associations between different haplotypes and MS risk were implemented with SNPStats online software (http://bioinfo.iconcologia.net/snpstats/start.htm) using the expectation–maximization (EM) algorithm27.

Results

IL-10 −1082 and −819 SNPs and their combinations with DRB1*15 allele were analysed in MS patients and controls to find any association between these genotypes and susceptibility to MS. At the −1082 position, G allele (P=0.001) and G/G genotype (P<0.001) were more frequent in MS patients than healthy controls (Table III). At the other polymorphic site, IL-10 −819, C allele had a higher frequency in MS patients compared with controls (P=0.09). The C/C genotype was significantly more frequent among patients (P=0.005) compared to controls (Table IV).

Table III Frequencies of interleukin-10 −1082 alleles and genotypes in patients and controls
Table IV Frequencies of interleukin-10 −819 alleles and genotypes in patients and controls

The association of the two SNPs with MS was estimated by calculating pooled OR and 95% CI under co-dominant, dominant and recessive genetic models using the SNPStats online software. In the IL-10 −1082 position, these genetic models compared A/A genotypes to G/A+G/G genotypes in dominant model and A/A+G/A to G/G genotypes in recessive model. The data are shown in Table V. DRB1*15 allele showed a higher frequency among MS patients as compared with control subjects (OR=1.6, CI=1.2-2.1, P=0.001).

Table V Association of interleukin-10 −1082 and interleukin-10 −819 genotypes with multiple sclerosis (MS) under different inheritance models (n=790, adjusted by age + sex)

Analysis of IL-10 polymorphisms and HLA-DRB alleles showed that some combinations were significantly more frequent among MS patients (Tables VI and VII). At the −1082 position, individuals having both G and DR15 alleles (determined as risk alleles) were at the highest risk for developing MS as compared with those who did not have these alleles (OR=3.7, CI=2.1-6.6, P<0.001). In addition, having one of these alleles was significantly associated with the risk of developing MS, compared with lacking both alleles.

Table VI Frequencies of risk alleles (interleukin-10 −1082 G and HLA-DRB1*1501) in multiple sclerosis (MS) patients and controls
Table VII Frequencies of risk alleles (interleukin-10 −819 C and HLA-DRB1*1501) in multiple sclerosis (MS) patients and controls

At the IL-10 −819 position, a significant difference was observed between patients and controls when both C and DR15 alleles were present simultaneously as compared with other combinations. Haplotype analysis with the EM (Expectation–Maximization) algorithm27 revealed a significantly higher occurrence of two haplotypes H2 (CA) and H4 (TG) in healthy controls than the MS patients (global haplotype association, P<0.001) (Table VIII), thereby suggesting protective roles for them against MS.

Table VIII Haplotype association with multiple sclerosis (MS) (n=790, adjusted by age + sex)

Discussion

This study evaluated IL-10 promoter polymorphisms and their combinations with HLA-DRB1*15 allele in Iranian MS patients and healthy controls. The results showed that −1082 G/G genotype and −819 CC genotype were associated with higher risk of developing MS. Together with HLA-DRB1*15 allele, IL-10 promoter SNPs and haplotypes were associated with susceptibility to MS.

Studies have shown that CD4+ T-cells are involved in the development of autoimmune diseases such as MS2829. Among CD4+ T-helper (Th) cell subsets, Th1 and Th17 CD4+ cells play a critical role in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), the most commonly used experimental model for human MS30. In contrast, regulatory T-cells and IL-10 have been shown to play a major role in protecting against and recovery from EAE31. Tullius et al29 showed that IL-10−/− mice were more susceptible to EAE when compared with their wild-type counterparts. These findings, in line with the previous studies, have shown that IL-10 production by interferon-γ-producing Th1 cells can prevent tissue damage and autoimmune diseases29.

Studies on human and animal models have shown that the amount of IL-10 production is closely correlated with disease course and also it differs in remission phase from relapse periods15. It is well known that the high production of IL-10 is associated with the suppression of inflammation in many autoimmune disorders including MS15. It has been shown that three SNPs in IL-10 promoter region, including −1082 (G/A), −819 (T/C) and −592 (A/C), regulate the expression of this cytokine17.

In the present study it was found that IL-10 −1082 G/G genotype was associated with higher risk of MS in Iranian population, while G/A and A/A genotypes reduced the risk of the disease. Luomala et al19 showed that −1082 SNP was not associated with the occurrence of MS, but it was associated with disease severity. They found that patients with G/A genotype suffered less severe disorder than other genotypes19. Mihailova et al18 studied IL-10 promoter polymorphisms in Bulgarian population and found that distribution of C/C genotype in −819 position was increased significantly in MS patients. They did not observe any significant difference in other genotype or allele frequencies between patients and controls18. In the present study, IL-10 polymorphisms were seen to be associated with susceptibility to MS against previous studies.

Several HLA allele groups have been a candidate as predisposing factor for MS. Among these, HLA-A, HLA-DRB and HLA-DQB1 alleles seem to be associated with susceptibility to MS11.

Among HLA-DRB alleles, HLA-DRB1*15 allele group seems to have the strongest association with MS12. Our study also confirms this fact. Hence, HLA alleles may influence role of other genes in the pathogenesis of MS32; lack of data about the effect of combination of IL-10 SNPs and HLA-DRB allele on MS indicates the need for such a study. The association between genetic polymorphisms and MS susceptibility may also vary with ethnicity. Racial and ethnic differences may affect not only susceptibility but also the phenotypic expression of MS, including clinical manifestations, site of lesions, disease course and prognosis33.

In conclusion, our data showed that gene-gene interaction of IL-10 and HLA-DRB1*15 polymorphisms might have an important role in the susceptibility to MS in Iranian population. This association may be due to ethnic differences and differences in the genetic background of the Iranian population. Further studies on other genetic polymorphisms may reveal other candidate genes associated with pathogenesis of MS and clarify complex interactions between genes and the environment.

Acknowledgment

Authors thank Arya Tina Gene (ATG) Biopharmaceutical Company, Gorgan, Iran for funding and the Golestan Blood Transfusion Centre for providing healthy controls.

Conflicts of Interest: None.

References

  1. , . Epidemiology of neuroimmunological diseases. J Neurol. 2006;253(Suppl 5):V2-8.
    [Google Scholar]
  2. , . The prevalence of multiple sclerosis in the world: An update. Neurol Sci. 2001;22:117-39.
    [Google Scholar]
  3. , . Multiple sclerosis in time and space – Geographic clues to cause. J Neurovirol. 2000;6(Suppl 2):S134-40.
    [Google Scholar]
  4. , , , , . Prevalence of multiple sclerosis in Isfahan, Iran. Neuroepidemiology. 2006;27:39-44.
    [Google Scholar]
  5. , , , , , , . Decreased interleukin-10 and increased interleukin-12p40 mRNA are associated with disease activity and characterize different disease stages in multiple sclerosis. Ann Neurol. 1999;45:695-703.
    [Google Scholar]
  6. , , , , , , . Risk for multiple sclerosis in dizygotic and monozygotic twins. Mult Scler. 2005;11:500-3.
    [Google Scholar]
  7. , , , , , , . Complex interactions among MHC haplotypes in multiple sclerosis: susceptibility and resistance. Hum Mol Genet. 2005;14:2019-26.
    [Google Scholar]
  8. , , , , , , . CD24 V/V is an allele associated with the risk of developing multiple sclerosis in the Spanish population. Mult Scler. 2006;12:511-4.
    [Google Scholar]
  9. , , , , , , . Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia. J Neurol Sci. 2008;267:62-5.
    [Google Scholar]
  10. , , , , , , . Parental transmission of HLA-DRB1*15 in multiple sclerosis. Hum Genet. 2008;122:661-3.
    [Google Scholar]
  11. International Multiple Sclerosis Genetics Consortium, Wellcome Trust Case Control Consortium. Genetic risk and a primary role for cell-mediated immune mechanisms in multiple sclerosis. Nature. 2011;476:214-9.
    [Google Scholar]
  12. , , , , , , . The relationship between HLA-DRB1 alleles and optic neuritis in Irish patients and the risk of developing multiple sclerosis. Br J Ophthalmol. 2007;91:1288-92.
    [Google Scholar]
  13. , , , . Gene polymorphisms of interleukins 1 and 10 in infectious and autoimmune diseases. Ann Med. 1998;30:469-73.
    [Google Scholar]
  14. , , , , . Association of interleukin-10 with hepatitis B virus (HBV) mediated disease progression in Indian population. Indian J Med Res. 2014;139:737-45.
    [Google Scholar]
  15. , , , . Insights into the immunopathogenesis of multiple sclerosis. Immunol Res. 2002;25:27-51.
    [Google Scholar]
  16. , , , , . Mapping of the human IL10 gene and further characterization of the 5’ flanking sequence. Immunogenetics. 1997;46:120-8.
    [Google Scholar]
  17. , , , , , , . Interleukin-10 promoter polymorphisms in patients with multiple sclerosis. J Neurol Sci. 2002;202:93-7.
    [Google Scholar]
  18. , , , , , , . Pro-and anti-inflammatory cytokine gene polymorphism profiles in Bulgarian multiple sclerosis patients. J Neuroimmunol. 2005;168:138-43.
    [Google Scholar]
  19. , , , , , , . Promoter polymorphism of IL-10 and severity of multiple sclerosis. Acta Neurol Scand. 2003;108:396-400.
    [Google Scholar]
  20. , , , , , , . CCR5-delta 32 allele is associated with the risk of developing multiple sclerosis in the Iranian population. Cell Mol Neurobiol. 2009;29:1205-9.
    [Google Scholar]
  21. , , , , , , . HLA-DRB1*1501 intensifies the impact of IL-6 promoter polymorphism on the susceptibility to multiple sclerosis in an Iranian population. Mult Scler. 2010;16:1173-7.
    [Google Scholar]
  22. , , , , , , . High frequency of the IL-2 -330 T/HLA-DRB1*1501 haplotype in patients with multiple sclerosis. Clin Immunol. 2010;137:134-8.
    [Google Scholar]
  23. , , , , . Interaction of HLA-DRB1*1501 allele and TNF-alpha -308 G/A single nucleotide polymorphism in the susceptibility to multiple sclerosis. Clin Immunol. 2011;139:277-81.
    [Google Scholar]
  24. , , , , , , . Recommended diagnostic criteria for multiple sclerosis: guidelines from the international panel on the diagnosis of multiple sclerosis. Ann Neurol. 2001;50:121-7.
    [Google Scholar]
  25. , , , , , , . Association between functional polymorphism in EGF gene and malignant melanoma. Lancet. 2002;359:397-401.
    [Google Scholar]
  26. , , , , , , . Analysis of HLA DR2&DQ6 (DRB1*1501, DQA1*0102, DQB1*0602) haplotypes in Iranian patients with multiple sclerosis. Cell Mol Neurobiol. 2009;29:109-14.
    [Google Scholar]
  27. , , , , , . SNPStats: A web tool for the analysis of association studies. Bioinformatics. 2006;22:1928-9.
    [Google Scholar]
  28. , , , , , , . Double positive CD4+CD8+ T cells: Key suppressive role in the production of autoantibodies in systemic lupus erythematosus. Indian J Med Res. 2014;140:513-9.
    [Google Scholar]
  29. , , , , , , . NAD+ protects against EAE by regulating CD4+ T-cell differentiation. Nat Commun. 2014;5:5101.
    [Google Scholar]
  30. , , , , , , . Cutting edge: the pathogenicity of IFN-γ-producing Th17 cells is independent of T-bet. J Immunol. 2013;190:4478-82.
    [Google Scholar]
  31. , , , , , , . IL-10 is critical in the regulation of autoimmune encephalomyelitis as demonstrated by studies of IL-10- and IL-4-deficient and transgenic mice. J Immunol. 1998;161:3299-306.
    [Google Scholar]
  32. , , , , . A NOTCH4 association with multiple sclerosis is secondary to HLA-DR*1501. Tissue Antigens. 2004;63:13-20.
    [Google Scholar]
  33. , , , , , , . Multiple sclerosis in US minority populations Clinical practice insights. Neurol Clin Pract. 2015;5:132-42.
    [Google Scholar]
Show Sections
Scroll to Top